Marzieh Aghababaie
University of Isfahan, Iran
Title: Covalent immobilization of Candida rugosa lipase on a novel enzymatic nanocomposite membrane prepared by covalent attachment of magnetic nanoparticle to poly acrylonitrile membrane
Biography
Biography: Marzieh Aghababaie
Abstract
Enzymatic nanocomposite membranes which are the combination of nanocomposite membranes and immobilized enzymes have received increasing attention, recently. In this study, poly acrylonitrile ultrafiltration membrane was aminated and then magnetic nanoparticles which were modified by glutaraldehyde or cyanuric chloride were covalently attached on the surface of membrane. Afterwards, Candida rugosa lipase (CRL) was covalently immobilized on this nanocomposite membrane. Nanoparticles and nanocomposite membrane were characterized with various techniques such as SEM, TEM, XRD, FTIR, ATR, AFM, contact angle goniometry and surface free energy measurement. The evidence of immobilization was also done by ATR, enzyme activity and loading efficiency. It was found that the PAN nanocomposite membrane increased the relative activity and loading capacity in comparison to UF membrane. The Km and vmax values represents the increasing of substrate affinity and decreasing of catalytic activity of immobilized enzyme due to the mass transfer limitation for both nanocomposite membranes. Thermal, storage, and operational stability of immobilized enzyme increased significantly which make it a suitable candidate for to bio-catalytic processes