Nor Hazwani Ahmad
Universiti Sains Malaysia, Malaysia
Title: Cytotoxicity study of silver nanoparticles synthesized by catharanthus roseus aqueous extract
Biography
Biography: Nor Hazwani Ahmad
Abstract
Interest in silver nanoparticles and their potential medical applications has increased in recent years due to its unique characteristics. The objective of the present study was to evaluate the cytotoxic effects of a green synthesis of silver nanoparticles (AgNPs) using Catharanthus roseus (C. roseus) aqueous extract on Jurkat (human acute T-cell leukemia) and HT29 (human colorectal adenocarcinoma) cell lines. The assays used were MTS [3-(4, 5-dimethylthiazol-2-yl)-5-(3-carbonxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium/phenazine metho sulfate], annexin V-FITC/propidium iodide, DNA fragmentation and cell cycle. The IC50 values obtained from MTS assay were 6.7 to 7.4 μg/ml for Jurkat cells while 13.0 to 13.5 μg/ml for HT29 cells at various incubations. Flow cytometric analysis demonstratedhigher percentages of early (annexin V-FITC+/PI-) and late (annexin V-FITC+/PI-) apoptotic cells in response to C. roseus-AgNPs, as compared to untreated cells. This is further confirmed by the detection of DNA fragments. The induction of apoptosis was associated with cell cycle arrest. These data indicated that C. roseus contains active compounds responsible for the AgNPs synthesis and its anticancer activity on Jurkat and HT29 cells, which can be applicable for therapeutic purposes.
