Disalvo Anibal
Universidad Nacional de Santiago del Estero, Argentina
Title: The interaction of nano-archeasomes with lung surfactant in monolayer biomimetic systems
Biography
Biography: Disalvo Anibal
Abstract
Archaeosomes (ARC) are lipid vesicles composed by polar archaeolipids (TPA) extracted from hyperhalopileachae bacteria. In an "in vivo" context, inhaled ARC must first interact with the pulmonary surfactants (PS) lining layer covering the internal surface of the alveolus. Interactions of ARCs with the PS film determine the subsequent retention and translocation of the inhaled ARC and hence their potential activity on target cells. As in the respiratory cycle, the surface pressure of the surfactant monolayer changes, interaction was measured at different initial surface pressures. The changes were pronounced at low surface pressure (10 mN/m), where ARCs produce a much more noticeable effect in comparison to than hydrogenated soy phosphatidylcholine liposomes (L). Moreover, ARC incorporation rate into the PS membrane was two-fold effective when they were added from the air phase above the monolayer in comparison to when they were injected in the aqueous subphase underneath it. This makes ARC excellent candidates for delivering drugs to the lungs as inhaled nanoliposomes since they appear to interact with the hydrophobic moiety of PS